› 3luc

X-ray diffraction
1.69Å resolution

Crystal structure of MID domain from hAGO2

Released:
DOI: 10.2210/pdb3luc/pdb
PDB entry 3luc coloured by chain, front view.
PDB entry 3luc coloured by chain, side view.
PDB entry 3luc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for 5'-nucleotide base-specific recognition of guide RNA by human AGO2.
Frank F, Sonenberg N, Nagar B
Nature 465 818-22 (2010)
PMID: 20505670

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute-2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 138 amino acids
Theoretical weight: 15.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKV8 (Residues: 439-575; Coverage: 16%)
Gene names: AGO2, EIF2C2
Sequence domains: Mid domain of argonaute
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PO4 8 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P1
Unit cell:
a: 39.986Å b: 46.766Å c: 66.437Å
α: 85.69° β: 72.2° γ: 83.86°
R-values:
R R work R free
0.176 0.174 0.21
Expression system: Escherichia coli

Quick links

Citations

117 review citations

The widespread regulation of microRNA biogenesis, function and decay.
Krol et al. (2010)
116 more

8 mentions without citation

The MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs.
Cora et al. (2014)
7 more