› 3ltq

X-ray diffraction
2.1Å resolution

Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag

Released:
DOI: 10.2210/pdb3ltq/pdb
PDB entry 3ltq coloured by chain, front view.
PDB entry 3ltq coloured by chain, side view.
PDB entry 3ltq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering encodable lanthanide-binding tags into loop regions of proteins.
Barthelmes K, Reynolds AM, Peisach E, Jonker HR, DeNunzio NJ, Allen KN, Imperiali B, Schwalbe H
J Am Chem Soc 133 808-19 (2011)
PMID: 21182275

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interleukin-1 beta Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01584 (Residues: 117-169, 170-269; Coverage: 57%)
Gene names: IL1B, IL1F2
Sequence domains: Interleukin-1 / 18
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

3 bound ligands:
Ligand TB 1 x TB
Ligand ACT 2 x ACT
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P6322
Unit cell:
a: 120.584Å b: 120.584Å c: 74.899Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Structure determination of protein-protein complexes with long-range anisotropic paramagnetic NMR restraints.
Hass et al. (2014)
7 more

5 mentions without citation

Engineering encodable lanthanide-binding tags into loop regions of proteins.
Barthelmes et al. (2011)
4 more