› 3lmh

X-ray diffraction
2Å resolution

Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with ADP

Released:
DOI: 10.2210/pdb3lmh/pdb
PDB entry 3lmh coloured by chain, front view.
PDB entry 3lmh coloured by chain, side view.
PDB entry 3lmh coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Crystal structure of the alpha-kinase domain of Dictyostelium myosin heavy chain kinase A.
Ye Q, Crawley SW, Yang Y, Côté GP, Jia Z
Sci Signal 3 ra17 (2010)
PMID: 20197546

Function and Biology Details

Reaction catalysed: 
ATP + [myosin heavy-chain] = ADP + [myosin heavy-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin heavy chain kinase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 34.61 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli
UniProt:
  • Canonical: P42527 (Residues: 552-841; Coverage: 25%)
Gene names: DDB_G0291231, mhckA, mhkA
Sequence domains: Alpha-kinase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand ZN 2 x ZN
Ligand PO4 2 x PO4
1 modified residue:
Ligand PHD 2 x PHD

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21212
Unit cell:
a: 82.824Å b: 110.047Å c: 78.714Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.259
Expression system: Escherichia coli

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Citations

6 review citations

Eukaryotic elongation factor 2 kinase as a drug target in cancer, and in cardiovascular and neurodegenerative diseases.
Liu et al. (2016)
5 more

6 mentions without citation

eEF2K Inhibitor Design: The Progression of Exemplary Structure-Based Drug Design.
Klupt et al. (2023)
5 more