› 3lj7

X-ray diffraction
2.3Å resolution

3D-crystal structure of humanized-rat fatty acid amide hydrolase (FAAH) conjugated with Carbamate inhibitor URB597

Released:
DOI: 10.2210/pdb3lj7/pdb
PDB entry 3lj7 coloured by chain, front view.
PDB entry 3lj7 coloured by chain, side view.
PDB entry 3lj7 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of fatty acid amide hydrolase bound to the carbamate inhibitor URB597: discovery of a deacylating water molecule and insight into enzyme inactivation.
Mileni M, Kamtekar S, Wood DC, Benson TE, Cravatt BF, Stevens RC
J Mol Biol 400 743-54 (2010)
PMID: 20493882

Function and Biology Details

Reaction catalysed: 
Anandamide + H(2)O = arachidonic acid + ethanolamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-161318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty-acid amide hydrolase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 573 amino acids
Theoretical weight: 63.11 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P97612 (Residues: 30-579; Coverage: 95%)
Gene names: Faah, Faah1
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain

Ligands and Environments

2 bound ligands:
Ligand OHO 2 x OHO
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 102.733Å b: 105.075Å c: 147.802Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.214
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The significance of G protein-coupled receptor crystallography for drug discovery.
Salon et al. (2011)
4 more

7 mentions without citation

Crystal structure of fatty acid amide hydrolase bound to the carbamate inhibitor URB597: discovery of a deacylating water molecule and insight into enzyme inactivation.
Mileni et al. (2010)
6 more