3lf4

X-ray diffraction
1.81Å resolution

Crystal Structure of Fluorescent Timer Precursor Blue102

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112323 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.43 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D1MPT3 (Residues: 6-71; Coverage: 28%)
Gene names: PAmCherry, PAmCherry1
Structure domains: Pantoate--beta-alanine Ligase; Chain: A,domain 2
Fluorescent protein Chain: B
Molecule details ›
Chain: B
Length: 164 amino acids
Theoretical weight: 18.9 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D1MPT3 (Residues: 72-236; Coverage: 70%)
Gene names: PAmCherry, PAmCherry1
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 107.368Å b: 42.344Å c: 84.964Å
α: 90° β: 127.06° γ: 90°
R-values:
R R work R free
0.215 0.213 0.257
Expression system: Escherichia coli