› 3lcp

X-ray diffraction
2.45Å resolution

Crystal structure of the carbohydrate recognition domain of LMAN1 in complex with MCFD2

Released:
DOI: 10.2210/pdb3lcp/pdb
PDB entry 3lcp coloured by chain, front view.
PDB entry 3lcp coloured by chain, side view.
PDB entry 3lcp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the LMAN1-CRD/MCFD2 transport receptor complex provides insight into combined deficiency of factor V and factor VIII.
Wigren E, Bourhis JM, Kursula I, Guy JE, Lindqvist Y
FEBS Lett 584 878-82 (2010)
PMID: 20138881

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155904 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein ERGIC-53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49257 (Residues: 32-277; Coverage: 51%)
Gene names: ERGIC53, F5F8D, LMAN1
Sequence domains: Legume-like lectin family
Structure domains: Jelly Rolls
Multiple coagulation factor deficiency protein 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 93 amino acids
Theoretical weight: 10.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NI22 (Residues: 58-146; Coverage: 74%)
Gene names: MCFD2, SDNSF
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P61
Unit cell:
a: 58.602Å b: 58.602Å c: 396.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.247
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The COPII pathway and hematologic disease.
Khoriaty et al. (2012)
3 more

1 mention without citation

Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport.
Satoh et al. (2020)