› 3k82

X-ray diffraction
1.4Å resolution

Crystal Structure of the third PDZ domain of PSD-95

Released:
DOI: 10.2210/pdb3k82/pdb
PDB entry 3k82 coloured by chain, front view.
PDB entry 3k82 coloured by chain, side view.
PDB entry 3k82 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Novel conformational aspects of the third PDZ domain of the neuronal post-synaptic density-95 protein revealed from two 1.4A X-ray structures.
Cámara-Artigas A, Murciano-Calles J, Gavira JA, Cobos ES, Martínez JC
J Struct Biol 170 565-9 (2010)
PMID: 20227506

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disks large homolog 4 Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 10.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P78352 (Residues: 305-402; Coverage: 14%)
Gene names: DLG4, PSD95
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand GOL 1 x GOL
Ligand EPE 1 x EPE
1 modified residue:
Ligand SNN 1 x SNN

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P43
Unit cell:
a: 42.98Å b: 42.98Å c: 46.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Contrasting roles of dynamics in protein allostery: NMR and structural studies of CheY and the third PDZ domain from PSD-95.
Lee AL. (2015)
1 more

4 mentions without citation

An oligomeric equilibrium intermediate as the precursory nucleus of globular and fibrillar supramacromolecular assemblies in a PDZ domain.
Murciano-Calles et al. (2010)
3 more