› 3jwi

X-ray diffraction
2.2Å resolution

Crystal structure analysis of the methyltransferase domain of bacterial-CtHen1-CN

Released:
DOI: 10.2210/pdb3jwi/pdb
PDB entry 3jwi coloured by chain, front view.
PDB entry 3jwi coloured by chain, side view.
PDB entry 3jwi coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Primary publication:
Structural and biochemical insights into 2'-O-methylation at the 3'-terminal nucleotide of RNA by Hen1.
Mui Chan C, Zhou C, Brunzelle JS, Huang RH
Proc Natl Acad Sci U S A 106 17699-704 (2009)
PMID: 19822745

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107078 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small RNA 2'-O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 24.22 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DJ37 (Residues: 259-465; Coverage: 45%)
Gene name: Cthe_2767
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 67.456Å b: 82.228Å c: 88.053Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.249
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Regulation of small RNA stability: methylation and beyond.
Ji et al. (2012)
5 more

2 mentions without citation

Structural and biochemical insights into 2'-O-methylation at the 3'-terminal nucleotide of RNA by Hen1.
Mui Chan et al. (2009)
1 more