› 3juh

X-ray diffraction
1.66Å resolution

Crystal structure of a mutant of human protein kinase CK2alpha with altered cosubstrate specificity

Released:
DOI: 10.2210/pdb3juh/pdb
PDB entry 3juh coloured by chain, front view.
PDB entry 3juh coloured by chain, side view.
PDB entry 3juh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inclining the purine base binding plane in protein kinase CK2 by exchanging the flanking side-chains generates a preference for ATP as a cosubstrate.
Yde CW, Ermakova I, Issinger OG, Niefind K
J Mol Biol 347 399-414 (2005)
PMID: 15740749

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 40.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68400 (Residues: 1-335; Coverage: 86%)
Gene names: CK2A1, CSNK2A1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 6 x CL
Ligand ANP 2 x ANP
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P43
Unit cell:
a: 71.368Å b: 71.368Å c: 126.467Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.218
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Protein kinases CK1 and CK2 as new targets for neurodegenerative diseases.
Perez et al. (2011)
1 more

2 mentions without citation

Structural and functional insights into the regulation mechanism of CK2 by IP6 and the intrinsically disordered protein Nopp140.
Lee et al. (2013)
1 more