› 3j41

Electron Microscopy
25Å resolution

Pseudo-atomic model of the Aquaporin-0/Calmodulin complex derived from electron microscopy

Released:
DOI: 10.2210/pdb3j41/pdb
PDB entry 3j41 coloured by chain, front view.
PDB entry 3j41 coloured by chain, side view.
PDB entry 3j41 coloured by chain, top view.
Source organisms:
Primary publication:
Allosteric mechanism of water-channel gating by Ca2+-calmodulin.
Reichow SL, Clemens DM, Freites JA, Németh-Cahalan KL, Heyden M, Tobias DJ, Hall JE, Gonen T
Nat Struct Mol Biol 20 1085-92 (2013)
PMID: 23893133
Related structures: EMD-5679

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143975 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lens fiber major intrinsic protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 263 amino acids
Theoretical weight: 28.24 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: Q6J8I9 (Residues: 1-263; Coverage: 100%)
Gene names: AQP0, MIP
Sequence domains: Major intrinsic protein
Calmodulin-1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 25Å
Relevant EMDB volumes: EMD-5679
Expression system: Escherichia coli BL21

Quick links

Citations

19 review citations

The ever changing moods of calmodulin: how structural plasticity entails transductional adaptability.
Villarroel et al. (2014)
18 more

10 mentions without citation

Aquaporin Protein-Protein Interactions.
Roche et al. (2017)
9 more