› 3isw

X-ray diffraction
2.8Å resolution

Crystal structure of filamin-A immunoglobulin-like repeat 21 bound to an N-terminal peptide of CFTR

Released:
DOI: 10.2210/pdb3isw/pdb
PDB entry 3isw coloured by chain, front view.
PDB entry 3isw coloured by chain, side view.
PDB entry 3isw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical basis of the interaction between cystic fibrosis transmembrane conductance regulator and immunoglobulin-like repeats of filamin.
Smith L, Page RC, Xu Z, Kohli E, Litman P, Nix JC, Ithychanda SS, Liu J, Qin J, Misra S, Liedtke CM
J Biol Chem 285 17166-76 (2010)
PMID: 20351101

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146639 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Filamin-A Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 10.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21333 (Residues: 2236-2329; Coverage: 4%)
Gene names: FLN, FLN1, FLNA
Sequence domains: Filamin/ABP280 repeat
Structure domains: Immunoglobulins
Cystic fibrosis transmembrane conductance regulator Chain: C
Molecule details ›
Chain: C
Length: 18 amino acids
Theoretical weight: 2.01 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P13569 (Residues: 5-22; Coverage: 1%)
Gene names: ABCC7, CFTR

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P6522
Unit cell:
a: 74.152Å b: 74.152Å c: 289.063Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.264 0.263 0.298
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

The filamins: organizers of cell structure and function.
Nakamura et al. (2011)
2 more

5 mentions without citation

Biochemical basis of the interaction between cystic fibrosis transmembrane conductance regulator and immunoglobulin-like repeats of filamin.
Smith et al. (2010)
4 more