› 3iqh

X-ray diffraction
1.9Å resolution

Structure of O-Acetylserine Sulfhydrylase in Complex with Peptide MNYDI

Released:
DOI: 10.2210/pdb3iqh/pdb
PDB entry 3iqh coloured by chain, front view.
PDB entry 3iqh coloured by chain, side view.
PDB entry 3iqh coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Design of O-acetylserine sulfhydrylase inhibitors by mimicking nature.
Salsi E, Bayden AS, Spyrakis F, Amadasi A, Campanini B, Bettati S, Dodatko T, Cozzini P, Kellogg GE, Cook PF, Roderick SL, Mozzarelli A
J Med Chem 53 345-56 (2010)
PMID: 19928859

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155238 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine synthase Chain: X
Molecule details ›
Chain: X
Length: 316 amino acids
Theoretical weight: 33.65 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45040 (Residues: 1-316; Coverage: 100%)
Gene names: HI_1103, cysK
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold
MNYDI Chain: P
Molecule details ›
Chain: P
Length: 5 amino acids
Theoretical weight: 655 Da
Source organism: Haemophilus influenzae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: I41
Unit cell:
a: 112.153Å b: 112.153Å c: 45.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.209
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

The cysteine regulatory complex from plants and microbes: what was old is new again.
Jez et al. (2013)
5 more

3 mentions without citation

Insights into multifaceted activities of CysK for therapeutic interventions.
Joshi et al. (2019)
2 more