› 3iix

X-ray diffraction
1.25Å resolution

X-ray structure of the FeFe-hydrogenase maturase HydE from T. maritima in complex with methionine and 5'deoxyadenosine

Released:
DOI: 10.2210/pdb3iix/pdb
PDB entry 3iix coloured by chain, front view.
PDB entry 3iix coloured by chain, side view.
PDB entry 3iix coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Unexpected electron transfer mechanism upon AdoMet cleavage in radical SAM proteins.
Nicolet Y, Amara P, Mouesca JM, Fontecilla-Camps JC
Proc Natl Acad Sci U S A 106 14867-71 (2009)
PMID: 19706452

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[FeFe] hydrogenase maturase subunit HydE Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.94 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X0Z6 (Residues: 1-348; Coverage: 100%)
Gene names: THEMA_07990, TM_1269, Tmari_1274
Sequence domains: Radical SAM superfamily
Structure domains: Aldolase class I

Ligands and Environments

7 bound ligands:
Ligand SF4 1 x SF4
Ligand MET 1 x MET
Ligand 5AD 1 x 5AD
Ligand CPS 5 x CPS
Ligand S3H 1 x S3H
Ligand CL 3 x CL
Ligand CO3 1 x CO3
2 modified residues:
Ligand CSO 2 x CSO
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 51.054Å b: 78.925Å c: 86.192Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.139 0.166
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Radical S-adenosylmethionine enzymes.
Broderick et al. (2014)
15 more

7 mentions without citation

Structural insights into radical generation by the radical SAM superfamily.
Vey et al. (2011)
6 more