› 3igk

X-ray diffraction
1.7Å resolution

Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs (p53-DNA complex 2)

Released:
DOI: 10.2210/pdb3igk/pdb
PDB entry 3igk coloured by chain, front view.
PDB entry 3igk coloured by chain, side view.
PDB entry 3igk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
Kitayner M, Rozenberg H, Rohs R, Suad O, Rabinovich D, Honig B, Shakked Z
Nat Struct Mol Biol 17 423-9 (2010)
PMID: 20364130

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116404 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 94-293; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
DNA (5'-D(*CP*GP*GP*GP*CP*AP*TP*GP*CP*CP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 137.298Å b: 49.624Å c: 33.879Å
α: 90° β: 94.72° γ: 90°
R-values:
R R work R free
0.153 0.15 0.214
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

21 review citations

The multiple mechanisms that regulate p53 activity and cell fate.
Hafner et al. (2019)
20 more

9 mentions without citation

Diversity in DNA recognition by p53 revealed by crystal structures with Hoogsteen base pairs.
Kitayner et al. (2010)
8 more