› 3ifj

X-ray diffraction
1.9Å resolution

Crystal structure of Mtu recA intein, splicing domain

Released:
DOI: 10.2210/pdb3ifj/pdb
PDB entry 3ifj coloured by chain, front view.
PDB entry 3ifj coloured by chain, side view.
PDB entry 3ifj coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Selection and structure of hyperactive inteins: peripheral changes relayed to the catalytic center.
Hiraga K, Soga I, Dansereau JT, Pereira B, Derbyshire V, Du Z, Wang C, Van Roey P, Belfort G, Belfort M
J Mol Biol 393 1106-17 (2009)
PMID: 19744499

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-161555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease PI-MtuI Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 15.41 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHJ3 (Residues: 252-691; Coverage: 18%)
Gene names: MTV002.02c, Rv2737c, recA
Sequence domains: Intein splicing domain
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand SNN 2 x SNN

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 39.81Å b: 86.56Å c: 92.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.26
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Interactions between Hedgehog proteins and their binding partners come into view.
Beachy et al. (2010)
5 more

2 mentions without citation

Selection and structure of hyperactive inteins: peripheral changes relayed to the catalytic center.
Hiraga et al. (2009)
1 more