› 3i9o

X-ray diffraction
3Å resolution

Crystal structure of ADP ribosyl cyclase complexed with ribo-2'F-ADP ribose

Released:
DOI: 10.2210/pdb3i9o/pdb
PDB entry 3i9o coloured by chain, front view.
PDB entry 3i9o coloured by chain, side view.
PDB entry 3i9o coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
Mechanism of cyclizing NAD to cyclic ADP-ribose by ADP-ribosyl cyclase and CD38.
Graeff R, Liu Q, Kriksunov IA, Kotaka M, Oppenheimer N, Hao Q, Lee HC
J Biol Chem 284 27629-36 (2009)
PMID: 19640843

Function and Biology Details

Reaction catalysed: 
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.58 KDa
Source organism: Aplysia californica
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P29241 (Residues: 25-282; Coverage: 100%)
Sequence domains: ADP-ribosyl cyclase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AVW 2 x AVW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P61
Unit cell:
a: 56.726Å b: 56.726Å c: 360.333Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.268
Expression system: Komagataella pastoris

Quick links

Citations

15 review citations

Sirtuins in Renal Health and Disease.
Morigi et al. (2018)
14 more

2 mentions without citation

Mechanism of cyclizing NAD to cyclic ADP-ribose by ADP-ribosyl cyclase and CD38.
Graeff et al. (2009)
1 more