› 3i9l

X-ray diffraction
1.75Å resolution

Crystal structure of ADP ribosyl cyclase complexed with N1-cIDPR

Released:
DOI: 10.2210/pdb3i9l/pdb
PDB entry 3i9l coloured by chain, front view.
PDB entry 3i9l coloured by chain, side view.
PDB entry 3i9l coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
Structural basis for enzymatic evolution from a dedicated ADP-ribosyl cyclase to a multifunctional NAD hydrolase.
Liu Q, Graeff R, Kriksunov IA, Jiang H, Zhang B, Oppenheimer N, Lin H, Potter BV, Lee HC, Hao Q
J Biol Chem 284 27637-45 (2009)
PMID: 19640846

Function and Biology Details

Reaction catalysed: 
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.51 KDa
Source organism: Aplysia californica
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P29241 (Residues: 25-282; Coverage: 100%)
Sequence domains: ADP-ribosyl cyclase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand N1C 1 x N1C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 69.627Å b: 58.303Å c: 71.651Å
α: 90° β: 101.37° γ: 90°
R-values:
R R work R free
0.167 0.166 0.196
Expression system: Komagataella pastoris

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Citations

13 review citations

The Multi-faceted Ecto-enzyme CD38: Roles in Immunomodulation, Cancer, Aging, and Metabolic Diseases.
Hogan et al. (2019)
12 more

2 mentions without citation

Structural basis for enzymatic evolution from a dedicated ADP-ribosyl cyclase to a multifunctional NAD hydrolase.
Liu et al. (2009)
1 more