› 3i27

X-ray diffraction
2Å resolution

Structure of bovine torovirus Hemagglutinin-Esterase in complex with receptor

Released:
DOI: 10.2210/pdb3i27/pdb
PDB entry 3i27 coloured by chain, front view.
PDB entry 3i27 coloured by chain, side view.
PDB entry 3i27 coloured by chain, top view.
Source organism: Breda virus serotype 1
Primary publication:
Structural basis for ligand and substrate recognition by torovirus hemagglutinin esterases.
Langereis MA, Zeng Q, Gerwig GJ, Frey B, von Itzstein M, Kamerling JP, de Groot RJ, Huizinga EG
Proc Natl Acad Sci U S A 106 15897-902 (2009)
PMID: 19721004

Function and Biology Details

Reaction catalysed: 
N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Hemagglutinin-esterase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 384 amino acids
Theoretical weight: 42.77 KDa
Source organism: Breda virus serotype 1
Expression system: Homo sapiens
UniProt:
  • Canonical: P0C0V9 (Residues: 15-392; Coverage: 94%)
Gene name: HE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 5 x NAG
Ligand SID 1 x SID
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 67.63Å b: 112.89Å c: 273.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.21
Expression system: Homo sapiens

Quick links

Citations

10 review citations

Viral quasispecies evolution.
Domingo et al. (2012)
9 more

3 mentions without citation

Viruses and sialic acids: rules of engagement.
Neu et al. (2011)
2 more