› 3huo

X-ray diffraction
1.5Å resolution

X-ray crystallographic structure of CTX-M-9 S70G in complex with benzylpenicillin

Released:
DOI: 10.2210/pdb3huo/pdb
PDB entry 3huo coloured by chain, front view.
PDB entry 3huo coloured by chain, side view.
PDB entry 3huo coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Delmas J, Leyssene D, Dubois D, Robin F, Bonnet R

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 27.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L5C8 (Residues: 29-291; Coverage: 100%)
Gene names: RCS51_P0174, bla CTX-M-9, blaCTX-M-9, blaCTX-M-9a, blaCTX-M-9b
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand PNN 5 x PNN
Ligand PNK 1 x PNK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 45.205Å b: 106.784Å c: 47.645Å
α: 90° β: 101.69° γ: 90°
R-values:
R R work R free
0.15 0.148 0.181
Expression system: Escherichia coli

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