› 3hue

X-ray diffraction
2.8Å resolution

Structure of the S. pombe Nbs1 FHA-BRCT1-BRCT2 domains

Released:
DOI: 10.2210/pdb3hue/pdb
PDB entry 3hue coloured by chain, front view.
PDB entry 3hue coloured by chain, side view.
PDB entry 3hue coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair.
Williams RS, Dodson GE, Limbo O, Yamada Y, Williams JS, Guenther G, Classen S, Glover JN, Iwasaki H, Russell P, Tainer JA
Cell 139 87-99 (2009)
PMID: 19804755

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair and telomere maintenance protein nbs1 Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 38.87 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O43070 (Residues: 1-330; Coverage: 54%)
Gene names: SPBC6B1.09c, nbs1
Sequence domains: FHA domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: C2221
Unit cell:
a: 54.671Å b: 204.538Å c: 62.897Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.285
Expression system: Escherichia coli

Quick links

Citations

83 review citations

Double-strand break end resection and repair pathway choice.
Symington et al. (2011)
82 more

7 mentions without citation

BRCT domains: easy as one, two, three.
Leung et al. (2011)
6 more