› 3hib

X-ray diffraction
2Å resolution

Crystal structure of the second Sec63 domain of yeast Brr2

Released:
DOI: 10.2210/pdb3hib/pdb
PDB entry 3hib coloured by chain, front view.
PDB entry 3hib coloured by chain, side view.
PDB entry 3hib coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2.
Zhang L, Xu T, Maeder C, Bud LO, Shanks J, Nix J, Guthrie C, Pleiss JA, Zhao R
Nat Struct Mol Biol 16 731-9 (2009)
PMID: 19525970

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-splicing helicase BRR2 Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 36.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32639 (Residues: 1851-2163; Coverage: 15%)
Gene names: BRR2, RSS1, SNU246, SYGP-ORF66, YER172C
Sequence domains: Sec63 Brl domain
Structure domains: Sec63 N-terminal domain-like domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 55.153Å b: 75.678Å c: 83.955Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.257
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Spliceosome structure and function.
Will et al. (2011)
20 more

5 mentions without citation

Ski2-like RNA helicase structures: common themes and complex assemblies.
Johnson et al. (2013)
4 more