› 3gw7

X-ray diffraction
3.3Å resolution

Crystal structure of a metal-dependent phosphohydrolase with conserved HD domain (yedJ) from Escherichia coli in complex with nickel ions. Northeast Structural Genomics Consortium Target ER63

Released:
DOI: 10.2210/pdb3gw7/pdb
PDB entry 3gw7 coloured by chain, front view.
PDB entry 3gw7 coloured by chain, side view.
PDB entry 3gw7 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Forouhar F, Abashidze M, Seetharaman J, Janjua J, Xiao R, Cunningham K, Ma L, Zhao L, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein yedJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P46144 (Residues: 1-231; Coverage: 100%)
Gene names: JW1945, b1962, yedJ
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

1 bound ligand:
Ligand NI 8 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P63
Unit cell:
a: 99.408Å b: 99.408Å c: 130.629Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.266 0.26 0.294
Expression system: Escherichia coli

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