› 3gr8

X-ray diffraction
2.5Å resolution

Structure of OYE from Geobacillus kaustophilus, orthorhombic crystal form

Released:
DOI: 10.2210/pdb3gr8/pdb
PDB entry 3gr8 coloured by chain, front view.
PDB entry 3gr8 coloured by chain, side view.
PDB entry 3gr8 coloured by chain, top view.
Source organism: Geobacillus kaustophilus
Primary publication:
Old Yellow Enzyme-Catalyzed Dehydrogenation of Saturated Ketones
Schittmayer M, Zach S, Winkler M, Winkler A, Macheroux P, Uhl MK, Gruber K, Kambourakis S, Rozzell D, Glieder A
Adv. Synth. Catal. 353 268-274 (2011)

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 37.73 KDa
Source organism: Geobacillus kaustophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KXG9 (Residues: 1-340; Coverage: 100%)
Gene names: GK2332, namA
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
Ligand SO4 1 x SO4
Ligand BTB 2 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I212121
Unit cell:
a: 82.394Å b: 151.93Å c: 143.744Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.213
Expression system: Escherichia coli

Quick links