› 3gr5

X-ray diffraction
2.05Å resolution

Periplasmic domain of the outer membrane secretin EscC from enteropathogenic E.coli (EPEC)

Released:
DOI: 10.2210/pdb3gr5/pdb
PDB entry 3gr5 coloured by chain, front view.
PDB entry 3gr5 coloured by chain, side view.
PDB entry 3gr5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A conserved structural motif mediates formation of the periplasmic rings in the type III secretion system.
Spreter T, Yip CK, Sanowar S, André I, Kimbrough TG, Vuckovic M, Pfuetzner RA, Deng W, Yu AC, Finlay BB, Baker D, Miller SI, Strynadka NC
Nat Struct Mol Biol 16 468-76 (2009)
PMID: 19396170

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 3 secretion system secretin Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: O52135 (Residues: 22-173; Coverage: 31%)
Gene names: escC, sctC
Sequence domains: SPI-1 type 3 secretion system secretin, N0 domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6522
Unit cell:
a: 90.645Å b: 90.645Å c: 133.422Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.251
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
33 more

17 mentions without citation

Secretins: dynamic channels for protein transport across membranes.
Korotkov et al. (2011)
16 more