› 3goq

X-ray diffraction
1.6Å resolution

Crystal structure of the Tyr13Met variant of Bacillus subtilis ferrochelatase

Released:
DOI: 10.2210/pdb3goq/pdb
PDB entry 3goq coloured by chain, front view.
PDB entry 3goq coloured by chain, side view.
PDB entry 3goq coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Karlberg T, Hansson MD, Hansson M, Al-Karadaghi S

Function and Biology Details

Reaction catalysed: 
Fe-coproporphyrin III + 2 H(+) = coproporphyrin III + Fe(2+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coproporphyrin III ferrochelatase Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 35.36 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P32396 (Residues: 1-310; Coverage: 100%)
Gene names: BSU10130, cpfC, hemF, hemH
Sequence domains: Ferrochelatase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-5
Spacegroup: P212121
Unit cell:
a: 48.33Å b: 49.9Å c: 118.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.232
Expression system: Escherichia coli

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