› 3gef

X-ray diffraction
1.5Å resolution

Crystal structure of the R482W mutant of lamin A/C

Released:
DOI: 10.2210/pdb3gef/pdb
PDB entry 3gef coloured by chain, front view.
PDB entry 3gef coloured by chain, side view.
PDB entry 3gef coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the lamin A/C R482W mutant responsible for dominant familial partial lipodystrophy (FPLD).
Magracheva E, Kozlov S, Stewart CL, Wlodawer A, Zdanov A
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 665-70 (2009)
PMID: 19574635

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lamin-A/C Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 118 amino acids
Theoretical weight: 13.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02545 (Residues: 436-552; Coverage: 18%)
Gene names: LMN1, LMNA
Sequence domains: Lamin Tail Domain
Structure domains: Lamin Tail domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSD 4 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 62.195Å b: 84.035Å c: 98.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.214 0.253
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Lamins: nuclear intermediate filament proteins with fundamental functions in nuclear mechanics and genome regulation.
Gruenbaum et al. (2015)
4 more

3 mentions without citation

Recent insight into intermediate filament structure.
Eldirany et al. (2021)
2 more