3gds

X-ray diffraction
2.85Å resolution

Crystal structure of DegS H198P/D320A mutant modified by DFP in complex with DNRDGNVYYF peptide

Released:

Function and Biology Details

Reaction catalysed:
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-142442 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine endoprotease DegS Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 36.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE3 (Residues: 27-355; Coverage: 93%)
Gene names: JW3204, b3235, degS, hhoB, htrH
Sequence domains:
Structure domains:
DNRDGNVYYF peptide Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.26 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P213
Unit cell:
a: 118.822Å b: 118.822Å c: 118.822Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.221
Expression systems:
  • Escherichia coli
  • Not provided