› 3gcn

X-ray diffraction
3Å resolution

Crystal structure of DegS H198P/D320A mutant modified by DFP in complex with OMP peptide (YQF)

Released:
DOI: 10.2210/pdb3gcn/pdb
PDB entry 3gcn coloured by chain, front view.
PDB entry 3gcn coloured by chain, side view.
PDB entry 3gcn coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Sohn J, Sauer RT, Grant RA

Function and Biology Details

Reaction catalysed: 
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142436 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine endoprotease DegS Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 36.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE3 (Residues: 27-355; Coverage: 93%)
Gene names: JW3204, b3235, degS, hhoB, htrH
Sequence domains:
Structure domains:
YQF peptide Chain: B
Molecule details ›
Chain: B
Length: 3 amino acids
Theoretical weight: 456 Da
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MIS 1 x MIS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P213
Unit cell:
a: 119.03Å b: 119.03Å c: 119.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.249
Expression systems:
  • Escherichia coli
  • Not provided

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