› 3gbf

X-ray diffraction
1.92Å resolution

Phpd with cadmium complexed with hydroethylphosphonate (HEP)

Released:
DOI: 10.2210/pdb3gbf/pdb
PDB entry 3gbf coloured by chain, front view.
PDB entry 3gbf coloured by chain, side view.
PDB entry 3gbf coloured by chain, top view.
Source organism: Streptomyces viridochromogenes
Entry authors: Zhang H, Nair SK

Function and Biology Details

Reaction catalysed: 
2-hydroxyethylphosphonate + O(2) = hydroxymethylphosphonate + formate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-hydroxyethylphosphonate dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 443 amino acids
Theoretical weight: 48.38 KDa
Source organism: Streptomyces viridochromogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5IW40 (Residues: 1-443; Coverage: 100%)
Gene names: SSQG_01041, hepD, phpD
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 2HE 1 x 2HE
Ligand CD 13 x CD
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 84.974Å b: 94.695Å c: 101.595Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.257
Expression system: Escherichia coli

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