› 3gax

X-ray diffraction
1.7Å resolution

Crystal structure of monomeric human cystatin C stabilized against aggregation

Released:
DOI: 10.2210/pdb3gax/pdb
PDB entry 3gax coloured by chain, front view.
PDB entry 3gax coloured by chain, side view.
PDB entry 3gax coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human cystatin C stabilized against amyloid formation.
Kolodziejczyk R, Michalska K, Hernandez-Santoyo A, Wahlbom M, Grubb A, Jaskolski M
FEBS J 277 1726-37 (2010)
PMID: 20175878

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystatin-C Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 13.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01034 (Residues: 27-146; Coverage: 100%)
Gene name: CST3
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P61
Unit cell:
a: 76.26Å b: 76.26Å c: 97.576Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.139 0.138 0.167
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Lysosomal cathepsins and their regulation in aging and neurodegeneration.
Stoka et al. (2016)
11 more

21 mentions without citation

The role of cysteine peptidases in coronavirus cell entry and replication: The therapeutic potential of cathepsin inhibitors.
Pišlar et al. (2020)
20 more