› 3g61

X-ray diffraction
4.35Å resolution

Structure of P-glycoprotein Reveals a Molecular Basis for Poly-Specific Drug Binding

Released:
DOI: 10.2210/pdb3g61/pdb
PDB entry 3g61 coloured by chain, front view.
PDB entry 3g61 coloured by chain, side view.
PDB entry 3g61 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding.
Aller SG, Yu J, Ward A, Weng Y, Chittaboina S, Zhuo R, Harrell PM, Trinh YT, Zhang Q, Urbatsch IL, Chang G
Science 323 1718-22 (2009)
PMID: 19325113

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent translocase ABCB1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1284 amino acids
Theoretical weight: 141.9 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P21447 (Residues: 1-1276; Coverage: 100%)
Gene names: Abcb1a, Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 2J8 4 x 2J8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 97.742Å b: 114.978Å c: 375.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.308 0.308 0.356
Expression system: Not provided

Quick links

Citations

248 review citations

Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature.
Arnison et al. (2013)
247 more

62 mentions without citation

Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes.
Lundbaek et al. (2010)
61 more