› 3g5v

X-ray diffraction
2Å resolution

Antibodies Specifically Targeting a Locally Misfolded Region of Tumor Associated EGFR

Released:
DOI: 10.2210/pdb3g5v/pdb
PDB entry 3g5v coloured by chain, front view.
PDB entry 3g5v coloured by chain, side view.
PDB entry 3g5v coloured by chain, top view.
Source organisms:
Primary publication:
Antibodies specifically targeting a locally misfolded region of tumor associated EGFR.
Garrett TP, Burgess AW, Gan HK, Luwor RB, Cartwright G, Walker F, Orchard SG, Clayton AH, Nice EC, Rothacker J, Catimel B, Cavenee WK, Old LJ, Stockert E, Ritter G, Adams TE, Hoyne PA, Wittrup D, Chao G, Cochran JR, Luo C, Lou M, Huyton T, Xu Y, Fairlie WD, Yao S, Scott AM, Johns TG
Proc Natl Acad Sci U S A 106 5082-7 (2009)
PMID: 19289842

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208214 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
806 light chain Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.33 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
808 heavy chain Chain: B
Molecule details ›
Chain: B
Length: 213 amino acids
Theoretical weight: 22.78 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Epidermal growth factor receptor Chain: C
Molecule details ›
Chain: C
Length: 16 amino acids
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00533 (Residues: 311-326; Coverage: 1%)
Gene names: EGFR, ERBB, ERBB1, HER1

Ligands and Environments

3 bound ligands:
Ligand ACT 2 x ACT
Ligand GOL 1 x GOL
Ligand FMT 3 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 35.925Å b: 83.161Å c: 72.214Å
α: 90° β: 92.43° γ: 90°
R-values:
R R work R free
0.229 0.226 0.279
Expression systems:
  • Mus musculus
  • Not provided

Quick links

Citations

11 review citations

Targeting the ERBB family in cancer: couples therapy.
Tebbutt et al. (2013)
10 more

5 mentions without citation

Antibodies specifically targeting a locally misfolded region of tumor associated EGFR.
Garrett et al. (2009)
4 more