› 3g3k

X-ray diffraction
1.24Å resolution

Crystal structure of the GluR6 ligand binding domain dimer I442H K494E K665R I749L Q753K E757Q mutant with glutamate and NaCl at 1.24 Angstrom resolution

Released:
DOI: 10.2210/pdb3g3k/pdb
PDB entry 3g3k coloured by chain, front view.
PDB entry 3g3k coloured by chain, side view.
PDB entry 3g3k coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Stability of ligand-binding domain dimer assembly controls kainate receptor desensitization.
Chaudhry C, Weston MC, Schuck P, Rosenmund C, Mayer ML
EMBO J 28 1518-30 (2009)
PMID: 19339989

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor ionotropic, kainate 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 29.42 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P42260 (Residues: 429-544, 667-806; Coverage: 29%)
Gene names: Glur6, Grik2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

4 bound ligands:
Ligand GLU 2 x GLU
Ligand CL 5 x CL
Ligand NA 2 x NA
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 51.133Å b: 113.844Å c: 52.098Å
α: 90° β: 115.22° γ: 90°
R-values:
R R work R free
0.147 0.146 0.165
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Emerging models of glutamate receptor ion channel structure and function.
Mayer ML. (2011)
5 more

1 mention without citation

Stability of ligand-binding domain dimer assembly controls kainate receptor desensitization.
Chaudhry et al. (2009)