› 3g01

X-ray diffraction
2.5Å resolution

Structure of GrC mutant E192R/E193G

Released:
DOI: 10.2210/pdb3g01/pdb
PDB entry 3g01 coloured by chain, front view.
PDB entry 3g01 coloured by chain, side view.
PDB entry 3g01 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of granzyme C reveals an unusual mechanism of protease autoinhibition.
Kaiserman D, Buckle AM, Van Damme P, Irving JA, Law RH, Matthews AY, Bashtannyk-Puhalovich T, Langendorf C, Thompson P, Vandekerckhove J, Gevaert K, Whisstock JC, Bird PI
Proc Natl Acad Sci U S A 106 5587-92 (2009)
PMID: 19299505

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Granzyme C Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.15 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P08882 (Residues: 21-247; Coverage: 99%)
Gene names: Ctla-5, Ctla5, Gzmc
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P61
Unit cell:
a: 71.375Å b: 71.375Å c: 207.171Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.212 0.236
Expression system: Komagataella pastoris

Quick links

Citations

8 review citations

Functional dissection of the granzyme family: cell death and inflammation.
Anthony et al. (2010)
7 more

3 mentions without citation

Broadly neutralizing antibodies target a haemagglutinin anchor epitope.
Guthmiller et al. (2022)
2 more