› 3fdm

X-ray diffraction
2.26Å resolution

alpha/beta foldamer in complex with Bcl-xL

Released:
DOI: 10.2210/pdb3fdm/pdb
PDB entry 3fdm coloured by chain, front view.
PDB entry 3fdm coloured by chain, side view.
PDB entry 3fdm coloured by chain, top view.
Source organisms:
Primary publication:
High-resolution structural characterization of a helical alpha/beta-peptide foldamer bound to the anti-apoptotic protein Bcl-xL.
Lee EF, Sadowsky JD, Smith BJ, Czabotar PE, Peterson-Kaufman KJ, Colman PM, Gellman SH, Fairlie WD
Angew Chem Int Ed Engl 48 4318-22 (2009)
PMID: 19229915

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163544 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2-like protein 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 158 amino acids
Theoretical weight: 17.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07817 (Residues: 1-209; Coverage: 66%)
Gene names: BCL2L, BCL2L1, BCLX
Sequence domains:
alpha/beta-peptide foldamer Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 17 amino acids
Theoretical weight: 1.75 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 11 x EDO
1 modified residue:
Ligand B3L 3 x B3L

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2221
Unit cell:
a: 73.161Å b: 92.281Å c: 165.668Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.172 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

16 review citations

Small molecules, big targets: drug discovery faces the protein-protein interaction challenge.
Scott et al. (2016)
15 more

5 mentions without citation

The Structural Biology of Bcl-xL.
Lee et al. (2019)
4 more