› 3ezw

X-ray diffraction
2Å resolution

Crystal Structure of a Hyperactive Escherichia coli Glycerol Kinase Mutant Gly230 --> Asp Obtained Using Microfluidic Crystallization Devices

Released:
DOI: 10.2210/pdb3ezw/pdb
PDB entry 3ezw coloured by chain, front view.
PDB entry 3ezw coloured by chain, side view.
PDB entry 3ezw coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of a hyperactive Escherichia coli glycerol kinase mutant Gly230 --> Asp obtained using microfluidic crystallization devices.
Anderson MJ, DeLabarre B, Raghunathan A, Palsson BO, Brunger AT, Quake SR
Biochemistry 46 5722-31 (2007)
PMID: 17441732

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141311 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol kinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 526 amino acids
Theoretical weight: 59.2 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6F3 (Residues: 2-501; Coverage: 100%)
Gene names: JW3897, b3926, glpK
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

4 bound ligands:
Ligand GOL 8 x GOL
Ligand CL 8 x CL
Ligand MG 1 x MG
Ligand EDO 19 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-3
Spacegroup: P21
Unit cell:
a: 91.116Å b: 114.26Å c: 212.624Å
α: 90° β: 91.15° γ: 90°
R-values:
R R work R free
0.168 0.167 0.225
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Determination of protein structures--a series of fortunate events.
Chruszcz et al. (2008)
2 more

3 mentions without citation

The FGGY carbohydrate kinase family: insights into the evolution of functional specificities.
Zhang et al. (2011)
2 more