› 3ezi

X-ray diffraction
1.7Å resolution

Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain without Ligand

Released:
DOI: 10.2210/pdb3ezi/pdb
PDB entry 3ezi coloured by chain, front view.
PDB entry 3ezi coloured by chain, side view.
PDB entry 3ezi coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural analysis of ligand stimulation of the histidine kinase NarX.
Cheung J, Hendrickson WA
Structure 17 190-201 (2009)
PMID: 19217390

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrate/nitrite sensor protein NarX Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 107 amino acids
Theoretical weight: 11.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFA2 (Residues: 42-148; Coverage: 18%)
Gene names: JW1213, b1222, narR, narX
Sequence domains: Type IV pili methyl-accepting chemotaxis transducer N-term
Structure domains: Histidine kinase NarX, sensor domain

Ligands and Environments

1 bound ligand:
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 28.241Å b: 39.822Å c: 83.947Å
α: 80.76° β: 89.57° γ: 85.29°
R-values:
R R work R free
0.189 0.189 0.239
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Evolution of two-component signal transduction systems.
Capra et al. (2012)
16 more

4 mentions without citation

Structural analysis of ligand stimulation of the histidine kinase NarX.
Cheung et al. (2009)
3 more