› 3erh

X-ray diffraction
2.4Å resolution

First structural evidence of substrate specificity in mammalian peroxidases: Crystal structures of substrate complexes with lactoperoxidases from two different species

Released:
DOI: 10.2210/pdb3erh/pdb
PDB entry 3erh coloured by chain, front view.
PDB entry 3erh coloured by chain, side view.
PDB entry 3erh coloured by chain, top view.
Source organism: Bubalus bubalis
Primary publication:
Structural evidence of substrate specificity in mammalian peroxidases: structure of the thiocyanate complex with lactoperoxidase and its interactions at 2.4 A resolution.
Sheikh IA, Singh AK, Singh N, Sinha M, Singh SB, Bhushan A, Kaur P, Srinivasan A, Sharma S, Singh TP
J Biol Chem 284 14849-56 (2009)
PMID: 19339248

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Lactoperoxidase Chain: A
Molecule details ›
Chain: A
Length: 595 amino acids
Theoretical weight: 67.9 KDa
Source organism: Bubalus bubalis
UniProt:
  • Canonical: A5JUY8 (Residues: 118-712; Coverage: 86%)
Gene name: LPO
Sequence domains: Animal haem peroxidase
Structure domains: Haem peroxidase domain superfamily, animal type

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
3 bound ligands:
Ligand SCN 2 x SCN
Ligand CA 1 x CA
Ligand IOD 7 x IOD
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 54.207Å b: 80.541Å c: 77.877Å
α: 90° β: 102.64° γ: 90°
R-values:
R R work R free
0.18 0.18 0.195

Quick links

Citations

3 review citations

Mode of action of lactoperoxidase as related to its antimicrobial activity: a review.
Bafort et al. (2014)
2 more

4 mentions without citation

Genomic differentiation between temperate and tropical Australian populations of Drosophila melanogaster.
Kolaczkowski et al. (2011)
3 more