› 3epc

Electron Microscopy
8Å resolution

CryoEM structure of poliovirus receptor bound to poliovirus type 1

Released:
DOI: 10.2210/pdb3epc/pdb
PDB entry 3epc coloured by chain, front view.
PDB entry 3epc coloured by chain, side view.
PDB entry 3epc coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of CD155 and electron microscopic studies of its complexes with polioviruses.
Zhang P, Mueller S, Morais MC, Bator CM, Bowman VD, Hafenstein S, Wimmer E, Rossmann MG
Proc Natl Acad Sci U S A 105 18284-9 (2008)
PMID: 19011098
Related structures: EMD-1570

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136702 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Poliovirus receptor Chain: R
Molecule details ›
Chain: R
Length: 213 amino acids
Theoretical weight: 23.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15151 (Residues: 30-242; Coverage: 54%)
Gene names: PVR, PVS
Sequence domains:
Capsid protein VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 283 amino acids
Theoretical weight: 31.46 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Escherichia coli
UniProt:
  • Canonical: P03300 (Residues: 599-881; Coverage: 13%)
Sequence domains: picornavirus capsid protein
Capsid protein VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 268 amino acids
Theoretical weight: 29.66 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Escherichia coli
UniProt:
  • Canonical: P03300 (Residues: 74-341; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Capsid protein VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 68 amino acids
Theoretical weight: 7.39 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Escherichia coli
UniProt:
  • Canonical: P03300 (Residues: 2-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
Capsid protein VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 235 amino acids
Theoretical weight: 26.24 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Escherichia coli
UniProt:
  • Canonical: P03300 (Residues: 342-576; Coverage: 11%)
Sequence domains: picornavirus capsid protein

Ligands and Environments

2 bound ligands:
Ligand SPH 1 x SPH
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8Å
Relevant EMDB volumes: EMD-1570
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Connections matter--how viruses use cell–cell adhesion components.
Mateo et al. (2015)
7 more

4 mentions without citation

Molecular determinants of enterovirus 71 viral entry: cleft around GLN-172 on VP1 protein interacts with variable region on scavenge receptor B 2.
Chen et al. (2012)
3 more