› 3eln

X-ray diffraction
1.42Å resolution

A Putative Fe2+-bound Persulfenate Intermediate in Cysteine Dioxygenase

Released:
DOI: 10.2210/pdb3eln/pdb
PDB entry 3eln coloured by chain, front view.
PDB entry 3eln coloured by chain, side view.
PDB entry 3eln coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A putative Fe2+-bound persulfenate intermediate in cysteine dioxygenase.
Simmons CR, Krishnamoorthy K, Granett SL, Schuller DJ, Dominy JE, Begley TP, Stipanuk MH, Karplus PA
Biochemistry 47 11390-2 (2008)
PMID: 18847220

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 23.06 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21816 (Residues: 1-200; Coverage: 100%)
Gene name: Cdo1
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE2 1 x FE2
Ligand 2CO 1 x 2CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P43212
Unit cell:
a: 57.523Å b: 57.523Å c: 122.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.133 0.177
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Dealing with methionine/homocysteine sulfur: cysteine metabolism to taurine and inorganic sulfur.
Stipanuk et al. (2011)
5 more

9 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
8 more