› 3ekl

X-ray diffraction
1.51Å resolution

Structural Characterization of tetrameric Mycobacterium tuberculosis fructose 1,6-bisphosphate aldolase - substrate binding and catalysis mechanism of a class IIa bacterial aldolase

Released:
DOI: 10.2210/pdb3ekl/pdb
PDB entry 3ekl coloured by chain, front view.
PDB entry 3ekl coloured by chain, side view.
PDB entry 3ekl coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural basis for catalysis of a tetrameric class IIa fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis.
Pegan SD, Rukseree K, Franzblau SG, Mesecar AD
J Mol Biol 386 1038-53 (2009)
PMID: 19167403

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-bisphosphate aldolase Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 37.28 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQA3 (Residues: 1-344; Coverage: 100%)
Gene names: MTCY13E10.25c, Rv0363c, fba
Sequence domains: Fructose-bisphosphate aldolase class-II
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand 13P 2 x 13P
Ligand ZN 2 x ZN
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: I222
Unit cell:
a: 61.285Å b: 120.227Å c: 164.823Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.176
Expression system: Escherichia coli

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Citations

3 review citations

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
2 more

3 mentions without citation

Structural basis for catalysis of a tetrameric class IIa fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis.
Pegan et al. (2009)
2 more