› 3ej5

X-ray diffraction
2.5Å resolution

complex of Ricin A chain and pyrimidine-based inhibitor

Released:
DOI: 10.2210/pdb3ej5/pdb
PDB entry 3ej5 coloured by chain, front view.
PDB entry 3ej5 coloured by chain, side view.
PDB entry 3ej5 coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
The X-ray structure of ricin A chain with a novel inhibitor.
Bai Y, Monzingo AF, Robertus JD
Arch Biochem Biophys 483 23-8 (2009)
PMID: 19138659

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: X
Molecule details ›
Chain: X
Length: 257 amino acids
Theoretical weight: 28.76 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02879 (Residues: 40-296; Coverage: 48%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EJ5 1 x EJ5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 42.642Å b: 68.006Å c: 49.675Å
α: 90° β: 112.97° γ: 90°
R-values:
R R work R free
0.198 0.194 0.266
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Ricinus communis intoxications in human and veterinary medicine-a summary of real cases.
Worbs et al. (2011)
4 more