› 3e74

X-ray diffraction
2.1Å resolution

Crystal structure of E. coli allantoinase with iron ions at the metal center

Released:
DOI: 10.2210/pdb3e74/pdb
PDB entry 3e74 coloured by chain, front view.
PDB entry 3e74 coloured by chain, side view.
PDB entry 3e74 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of metal-dependent allantoinase from Escherichia coli.
Kim K, Kim MI, Chung J, Ahn JH, Rhee S
J Mol Biol 387 1067-74 (2009)
PMID: 19248789

Function and Biology Details

Reaction catalysed: 
(S)-allantoin + H(2)O = allantoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160188 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Allantoinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 473 amino acids
Theoretical weight: 52.6 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77671 (Residues: 1-453; Coverage: 100%)
Gene names: JW0500, allB, b0512, glxB3, ybbX
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FE 8 x FE
2 modified residues:
Ligand MSE 52 x MSE
Ligand KCX 4 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P21
Unit cell:
a: 83.681Å b: 92.45Å c: 168.43Å
α: 90° β: 93.3° γ: 90°
R-values:
R R work R free
0.199 0.199 0.24
Expression system: Escherichia coli

Quick links

Citations

16 citation in other articles

Chemical rescue of the post-translationally carboxylated lysine mutant of allantoinase and dihydroorotase by metal ions and short-chain carboxylic acids.
Ho et al. (2013)
15 more

7 mentions without citation

Reversible post-translational carboxylation modulates the enzymatic activity of N-acetyl-L-ornithine transcarbamylase.
Li et al. (2010)
6 more