› 3e73

X-ray diffraction
2.8Å resolution

Crystal Structure of Human LanCL1 complexed with GSH

Released:
DOI: 10.2210/pdb3e73/pdb
PDB entry 3e73 coloured by chain, front view.
PDB entry 3e73 coloured by chain, side view.
PDB entry 3e73 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human lanthionine synthetase C-like protein 1 and its interaction with Eps8 and glutathione.
Zhang W, Wang L, Liu Y, Xu J, Zhu G, Cang H, Li X, Bartlam M, Hensley K, Li G, Rao Z, Zhang XC
Genes Dev 23 1387-92 (2009)
PMID: 19528316

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase LANCL1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 411 amino acids
Theoretical weight: 46.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O43813 (Residues: 1-399; Coverage: 100%)
Gene names: GPR69A, LANCL1
Sequence domains: Lanthionine synthetase C-like protein
Structure domains: Glycosyltransferase

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P6522
Unit cell:
a: 194.1Å b: 194.101Å c: 243.554Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.231 0.262
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Emerging biological importance of central nervous system lanthionines.
Hensley et al. (2010)
4 more

5 mentions without citation

Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes.
Repka et al. (2017)
4 more