› 3e4w

X-ray diffraction
1.8Å resolution

Crystal structure of a 33kDa catalase-related protein from Mycobacterium avium subsp. paratuberculosis. P2(1)2(1)2(1) crystal form.

Released:
DOI: 10.2210/pdb3e4w/pdb
PDB entry 3e4w coloured by chain, front view.
PDB entry 3e4w coloured by chain, side view.
PDB entry 3e4w coloured by chain, top view.
Source organism: Mycobacterium avium subsp. paratuberculosis
Primary publication:
The structure and peroxidase activity of a 33-kDa catalase-related protein from Mycobacterium avium ssp. paratuberculosis.
Pakhomova S, Gao B, Boeglin WE, Brash AR, Newcomer ME
Protein Sci 18 2559-68 (2009)
PMID: 19827095

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180977 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase-related peroxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 34.32 KDa
Source organism: Mycobacterium avium subsp. paratuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q73WB6 (Residues: 1-313; Coverage: 100%)
Gene name: MAP_2744c
Sequence domains: Catalase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand HEM 2 x HEM
Ligand HEZ 6 x HEZ
Ligand PO4 5 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: P212121
Unit cell:
a: 68.721Å b: 81.62Å c: 163.108Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.21
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Thirty years of heme catalases structural biology.
Díaz et al. (2012)
6 other articles

2 mentions without citation

The structure and peroxidase activity of a 33-kDa catalase-related protein from Mycobacterium avium ssp. paratuberculosis.
Pakhomova et al. (2009)
1 more