› 3e3j

X-ray diffraction
6.7Å resolution

Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA

Released:
DOI: 10.2210/pdb3e3j/pdb
PDB entry 3e3j coloured by chain, front view.
PDB entry 3e3j coloured by chain, side view.
PDB entry 3e3j coloured by chain, top view.
Source organisms:
Primary publication:
The structure of a transcribing T7 RNA polymerase in transition from initiation to elongation.
Durniak KJ, Bailey S, Steitz TA
Science 322 553-7 (2008)
PMID: 18948533

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116193 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chains: B, C
Molecule details ›
Chains: B, C
Length: 889 amino acids
Theoretical weight: 99.83 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
DNA (28-MER) Chains: T, X
Molecule details ›
Chains: T, X
Length: 32 nucleotides
Theoretical weight: 9.79 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*DTP*DAP*DAP*DTP*DAP*DCP*DGP*DAP*DCP*DTP*DCP*DAP*DCP*DTP*DAP*DTP*DAP*DTP*DTP*DTP*DCP*DTP*DGP*DCP*DCP*DAP*DAP*DAP*DCP*DGP*DGP*DC)-3') Chains: N, Y
Molecule details ›
Chains: N, Y
Length: 32 nucleotides
Theoretical weight: 9.74 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*GP*GP*AP*GP*UP*AP*A)-3') Chains: R, Z
Molecule details ›
Chains: R, Z
Length: 8 nucleotides
Theoretical weight: 2.63 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 99.216Å b: 180.602Å c: 198.619Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.395 0.392 0.454
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Structural basis of transcription elongation.
Martinez-Rucobo et al. (2013)
8 more

3 mentions without citation

Computational simulation strategies for analysis of multisubunit RNA polymerases.
Wang et al. (2013)
2 more