› 3e2h

X-ray diffraction
3.8Å resolution

Structure of the m67 high-affinity mutant of the 2C TCR in complex with Ld/QL9

Released:
DOI: 10.2210/pdb3e2h/pdb
PDB entry 3e2h coloured by chain, front view.
PDB entry 3e2h coloured by chain, side view.
PDB entry 3e2h coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Distinct CDR3 conformations in TCRs determine the level of cross-reactivity for diverse antigens, but not the docking orientation.
Jones LL, Colf LA, Stone JD, Garcia KC, Kranz DM
J Immunol 181 6255-64 (2008)
PMID: 18941216

Function and Biology Details

Reaction catalysed: 
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-106901 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class I histocompatibility antigen, L-D alpha chain Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 20.54 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01897 (Residues: 25-199; Coverage: 52%)
Gene name: H2-L
Sequence domains: Class I Histocompatibility antigen, domains alpha 1 and 2
Structure domains: MHC class I-like antigen recognition-like
2-oxoglutarate dehydrogenase complex component E1 Chain: Q
Molecule details ›
Chain: Q
Length: 9 amino acids
Theoretical weight: 1.06 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q60597 (Residues: 932-940; Coverage: 1%)
Gene names: Kiaa4192, Ogdh
T-cell receptor alpha chain V region PHDS58 Chain: B
Molecule details ›
Chain: B
Length: 109 amino acids
Theoretical weight: 12.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01738 (Residues: 22-130; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 110 amino acids
Theoretical weight: 11.91 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A2NTY6 (Residues: 31-144; Coverage: 96%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P6422
Unit cell:
a: 112.603Å b: 112.603Å c: 272.451Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

T-cell receptor binding affinities and kinetics: impact on T-cell activity and specificity.
Stone et al. (2009)
6 more

4 mentions without citation

Distinct CDR3 conformations in TCRs determine the level of cross-reactivity for diverse antigens, but not the docking orientation.
Jones et al. (2008)
3 more