› 3dnt

X-ray diffraction
1.66Å resolution

structures of MDT proteins

Released:
DOI: 10.2210/pdb3dnt/pdb
PDB entry 3dnt coloured by chain, front view.
PDB entry 3dnt coloured by chain, side view.
PDB entry 3dnt coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB.
Schumacher MA, Piro KM, Xu W, Hansen S, Lewis K, Brennan RG
Science 323 396-401 (2009)
PMID: 19150849

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase toxin HipA Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 49.87 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P23874 (Residues: 1-440; Coverage: 100%)
Gene names: JW1500, b1507, hipA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 4 x MG
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 68.54Å b: 84.09Å c: 69.25Å
α: 90° β: 91.56° γ: 90°
R-values:
R R work R free
0.184 0.184 0.217
Expression system: Escherichia coli

Quick links

Citations

72 review citations

Persister cells.
Lewis K. (2010)
71 more

4 mentions without citation

Wake me when it's over - Bacterial toxin-antitoxin proteins and induced dormancy.
Coussens et al. (2016)
3 more