› 3djh

X-ray diffraction
1.25Å resolution

Macrophage Migration Inhibitory Factor (MIF) at 1.25 A Resolution

Released:
DOI: 10.2210/pdb3djh/pdb
PDB entry 3djh coloured by chain, front view.
PDB entry 3djh coloured by chain, side view.
PDB entry 3djh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and kinetic analyses of macrophage migration inhibitory factor active site interactions.
Crichlow GV, Lubetsky JB, Leng L, Bucala R, Lolis EJ
Biochemistry 48 132-9 (2009)
PMID: 19090677

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

3 bound ligands:
Ligand SO4 6 x SO4
Ligand GOL 4 x GOL
Ligand IPA 3 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 68.232Å b: 68.255Å c: 86.512Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 0.186
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The modern pharmacology of paracetamol: therapeutic actions, mechanism of action, metabolism, toxicity and recent pharmacological findings.
Graham et al. (2013)
2 more

23 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
22 more