› 3die

X-ray diffraction
1.85Å resolution

Domain swapping of Staphylococcus Aureus thioredoxin W28A mutant

Released:
DOI: 10.2210/pdb3die/pdb
PDB entry 3die coloured by chain, front view.
PDB entry 3die coloured by chain, side view.
PDB entry 3die coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Coupling of domain swapping to kinetic stability in a thioredoxin mutant.
Garcia-Pino A, Martinez-Rodriguez S, Wahni K, Wyns L, Loris R, Messens J
J Mol Biol 385 1590-9 (2009)
PMID: 19071139

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.56 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0K6 (Residues: 1-104; Coverage: 100%)
Gene name: trxA
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand CD 4 x CD
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21212
Unit cell:
a: 88.703Å b: 84.451Å c: 34.699Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.22
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure, function, and mechanism of thioredoxin proteins.
Collet et al. (2010)
2 more

6 mentions without citation

Relative solvent accessible surface area predicts protein conformational changes upon binding.
Marsh et al. (2011)
5 more